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David further analyzed 71 target genes, the prediction of biological processes (BP), including protein phosphorylation (GO:0006468), positive regulation of cell proliferation (GO:0008284), peptidyl-serine phosphorylation (GO:0018105), Positive regulation of gene expression (GO:0010628), negative regulation of apoptotic process (GO:0043066), response to lipopolysaccharide (GO:0032496), positive regulation of MAPK cascade (GO:0043410), positive regulation of MAP kinase activity (GO:0043406) (Figure 11D)

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26 Sirtuins manipulate the metabolism reprogramming via directedly altering the activity of metabolic enzymes by NAD + -dependent modification or changing their expression by regulating transcription factors