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With this addition, glutathione’s role in mitigating toxicity from quinone-derivatives is combined with its overall kinetics providing a link between glutathione depletion in different compartments with quinone redox
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Tissues were homogenized in RIPA buffer (Tris (50 mM), NaCl (150 mM), Triton X-100 (1%), disodium EDTA (1 mM), SDS (0.1%), sodium deoxycholate (0.5%)) containing protease and phosphatase inhibitors (S8830, SigmaFast, Thermo Scientific) followed with mild sonication (5 min, cycle: 30 s on/off