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The experimental study on mice have shown the positive regulation of Sult1e1 , Phlda1 , and Ciart genes in the liver with vitamin U food supplementation, suggesting that vitamin U may regulate xenobiotic, glucose, and circadian rhythm pathways (Egea et al., 2025)

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The libraries were then prepared through end repair, A-tailing, adapter ligation, size selection, PCR amplification and purification