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GPx-1 overexpression, however, is protective against apoptosis only in circumstances where there is a disruption in normal redox balance favoring oxidation ( i.e ., under conditions of oxidative stress)

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In addition, a GSH/GSSG assay kit was used in this study, which is capable of accurately quantifying GSH/GSSG in cells by utilizing the enzymatic reaction between 5, 5′-dithiobis-2-nitrobenzoic acid (DTNB) and glutathione reductase (GR)