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Furthermore, HRMS-based non-targeted analyses using MS/MS structural elucidation allowed to identify other relatively minor metabolites related to APAP-Cys such as S-(5-acetylamino-2-hydroxyphenyl)mercaptopyruvic acid (SAMP), an APAP metabolite potentially formed by the transamination reaction of APAP-cysteine (see Fig.1), previously observed in mouse ( 29 )

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It interacts with multiple systems in the body — including the nitric oxide (NO) pathway, growth hormone receptors, and angiogenic regulators like VEGF (vascular endothelial growth factor) — to promote tissue repair, reduce oxidative stress, and modulate the immune response