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The membranes were blocked in QuickBlock™ Blocking Buffer for 15 min (Beyotime) and then probed with primary antibodies against fibronectin, collagen I, GFAP, α-SMA, TGFβ1, SMAD3, phospho-Smad3 (Thr8), SMAD4, α-tubulin, and β-actin (Supplementary Table 2)

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