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The observed accumulation of GSH in Sp treated cells was probably due to reduced activity of γ-glutamyl transpeptidase (GGT), an enzyme responsible for glutathione catabolism 36

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In antioxidant defense system, exogenous polyamines improved GSH level and GSH/(GSSG, glutathione dissulfide) ratio, activities of GR and GPX which defended overproduction of ROS