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10 mM NaF, 1 mM Sodium orthovanadate, 1 mM PMSF, 1× cOmplete Mini (Roche, Mannheim, Germany), 0.3 µg/mL Trichostatin A, 10 mM Nicotinamide, 5 mM Beta-glycerophosphoric acid, 1 mM dithiothreitol (DTT)) for 30 min on ice